One Test brings you One Step Closer to Compliance.
Order DOT-compliant drug tests with ease. Whether pre-employment, random, or return-to-duty, we’ve got you covered with fast scheduling, nationwide collection sites, and certified results.
Have questions about the process or want to consult before ordering? Our team is happy to help you understand every step.
Contact Information
Industry Overview
Under FMCSA regulations (49 CFR Part 382), CDL drivers in safety-sensitive positions must be enrolled in a drug and alcohol testing program. DOT drug testing is required for the events below.
- Pre-Employment
– Before a CDL driver performs any safety-sensitive function.
- Random Selection
– Ongoing throughout the year at federally mandated selection rates (currently 50% drug / 10% alcohol).
- Post-Accident
– After qualifying DOT-reportable accidents based on fatalities, citations, or tow-aways.
- Reasonable Suspicion
– When a trained supervisor observes possible drug or alcohol use (behavior, appearance, speech, or odor).
- Return-to-Duty
– Before a driver resumes safety-sensitive duties after a drug/alcohol violation and SAP process.
- Follow-Up (SAP)
– As prescribed and scheduled by a Substance Abuse Professional (SAP) following return-to-duty.
Failing to comply with DOT drug testing requirements can result in out-of-service orders, fines, and failed audits. Our service ensures you’re covered at every stage.
⚠️ Current FMCSA random selection rates: 50% Drug / 10% Alcohol. Failure to test triggers enforcement risk.
Maintain policies, selection logs, MRO-reviewed results, and MIS data readiness to pass audits and protect your DOT authority.
Pricing
Simple, transparent pricing for one-off tests or managed programs.
DOT Drug Test
$55
Per test (all standard DOT reasons)
Includes scheduling support, nationwide collection, chain-of-custody handling, and certified MRO review.
Out-of-network or after-hours collections (if requested) may incur a pass-through fee. Contact us for bulk & multi-location pricing.
No-Hassle Ordering Advantages
Easy Online Ordering
Place your order in minutes with a simple guided form.
Auto-Populated Donor Info
Upload a license photo and we populate key fields to reduce errors.
Nationwide Coverage
10,000+ compliant collection sites across the U.S.
Certified MRO Review
Every lab result is reviewed and verified for accuracy.
Secure Digital Delivery
PDF results archived and ready for your DQ file.
All Test Reasons Supported
Pre‑Employment, Random, Post‑Accident, RTD, Follow-Up, & more.
Why Take DOT Drug Testing Seriously?
Drug and alcohol violations are one of the top reasons carriers lose their authority. Beyond fines and shutdowns, employing an impaired driver exposes you to severe liability, audit failures, lost contracts, and higher insurance premiums. DOT-compliant testing isn’t just a rule—it’s critical protection for your business, your drivers, and everyone on the road.
Severe Liability in Accidents – A single impaired driving incident can devastate your business.
Audit Failures – Missing test records or policies leads to failed FMCSA safety audits.
Lost Contracts – Shippers and brokers demand proof of compliance before doing business.
Insurance Consequences – Violations can drive up premiums or cancel coverage.
What Happens After You Order
Place Order
Specify the driver and test reason (pre-employment, random, post-accident, RTD, etc.). Also, acknowledge the method using which results will be delivered (e.g., email).
Scheduling Link Sent
Driver receives a link, clicks it to schedule the test at their convenience.
Collection & Lab Processing
Specimen collected under 49 CFR Part 40 chain-of-custody; forwarded to certified lab.
MRO Review
Medical Review Officer validates results, contacts donor if needed for prescriptions.
Results Delivered
Secure electronic reporting (negatives typically 24–48 hrs). Store in DQ file.
Compliance Follow-Up (If Needed)
Positive / refusal: SAP referral & Return-to-Duty + Follow-Up test scheduling & tracking.
Typical Turnaround: Negatives in 24–48 hours after collection. Delays may occur if MRO must contact donor.
DOT Drug Testing vs. Non-DOT Drug Testing
| DOT-Compliant Drug Test | Non-DOT Drug Test |
|---|---|
| ✔ Follows strict FMCSA 49 CFR Part 40 regulations | ❌ May not follow federal testing procedures |
| ✔ Required for CDL drivers in safety-sensitive roles | ❌ Typically used for non-regulated employees |
| ✔ Uses 5-panel test (marijuana, cocaine, amphetamines, opioids, PCP) | ❌ Panels vary; may include different or fewer substances |
| ✔ Collection at certified sites only | ❌ May allow non-certified labs or at-home kits |
| ✔ Reviewed by a certified MRO (Medical Review Officer) | ❌ Often no MRO review — results less defensible |
| ✔ Results accepted for FMCSA compliance, audits, and Clearinghouse | ❌ Not valid for DOT compliance requirements |
| ✔ Protects your authority, CSA score, and contracts | ❌ Cannot be used to meet DOT requirements |
Bottom line: Only DOT-compliant tests keep trucking companies legal and audit-ready.
Contact Us
Need help deciding or have a compliance question? Reach out—our team answers real startup and audit questions every day.